Juan G. Cueva, Atticus Mulholland, and Miriam B. Goodman
Cueva et al. wanted to see just how mechano-electrical transduction (MeT) channels open in Caenorhabditis elegans. The prevailing idea is that 15-protofilament microtubules and an electrondense extracellular matrix serve as gating tethers to open these channels in touch receptor neurons. To look closer, the authors used high-pressure freezing and serial-section immunoelectron microscopy. Antibodies against MeT channel subunits showed distinct puncta along the processes of touch receptor neurons. These puncta were in distinct, but overlapping, domains compared to collagen associated with the putative microtubular gating tethers. Many channel puncta were >5 nm from the nearest filament. However, the specialized microtubules were assembled in a cross-linked bundle that was connected by kinked filaments to the cell membrane. The authors suggest that this assembly converts external pressure into membrane stretch and thus facilitates MeT channel openings.
Early Auditory Circuit Assembly
Edmund J. Koundakjian, Jessica L. Appler, and Lisa V. Goodrich
Cochlear ganglion precursors in the otic vesicle express the transcription factor Neurogenin1 (Ngn1). Thus, Koundakjian et al. generated a transgenic mouse that expressed Ngn1-driven, tamoxifeninducible Cre recombinase so that they could follow the early development of cochlear ganglion neurons. Using low-dose tamoxifen to induce only sparse labeling with Cre, the authors identified isolated clusters (
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